1LAF

STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	37.790, 59.630, 115.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.060
R-factor	0.161
Rwork	0.161
RMSD bond length	0.014
RMSD bond angle	1.290 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.060 *
Rmerge	0.051 *
Completeness [%]	82.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	sodium acetate	0.1 (M)	precipitant
3	1	drop	sodium cacodylate	0.05 (M)	precipitant
4	1	drop	PEG4000	25 (%)	precipitant
5	1	reservoir	precipitant		20 ml