1L9X
Structure of gamma-Glutamyl Hydrolase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-03 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.00918, 1.00870, 1.0064, 0.9918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.763, 156.451, 161.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 * - 1.600 |
| R-factor | 0.182 * |
| Rwork | 0.181 |
| R-free | 0.20200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.045 | 0.211 |
| Number of reflections | 161520 | |
| <I/σ(I)> | 53 | 4.4 |
| Completeness [%] | 82.2 | 31.5 |
| Redundancy | 6.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | PEG4000, ammonium acetate, sodium chloride, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 288.K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 9-13 (%) | |
| 2 | 1 | reservoir | ammonium acetate | 0.05-0.2 (M) | |
| 3 | 1 | reservoir | 0.1-0.2 (M) | ||
| 4 | 1 | reservoir | sodium citrate | 0.08 (M) | pH5.6 |
| 5 | 1 | drop | protein | 3.5 (mg/ml) | |
| 6 | 1 | drop | 50 (mM) | ||
| 7 | 1 | drop | beta-mercaptoethanol | 50 (mM) | |
| 8 | 1 | drop | octyl beta-glucopyranoside | 1 (mM) | |
| 9 | 1 | drop | 500 (mM) | pH5.5 |
