1L9S
CRYSTAL STRUCTURE OF THE I257T VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIS S-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.480, 102.030, 146.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.540 - 1.780 |
| Rwork | 0.157 |
| R-free | 0.19700 |
| Structure solution method | isomorphous |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.540 | 1.890 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.066 | 0.274 |
| Number of reflections | 77116 | |
| <I/σ(I)> | 15.1 | 4.1 |
| Completeness [%] | 87.2 | 71.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
