1L9P
CRYSTAL STRUCTURE OF NITRITE SOAKED I257G VARIANT OF THE COPPER-CONTAINING NITRITE REDUCTASE FROM ALCALIGENES FAECALIES S-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-19 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.050, 102.450, 146.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.610 - 1.750 |
| Rwork | 0.159 |
| R-free | 0.19400 |
| Structure solution method | isomorphous |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.610 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.057 | 0.324 |
| Number of reflections | 92925 | |
| <I/σ(I)> | 19.7 | 2.8 |
| Completeness [%] | 97.0 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | acetate peg 4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
