1L9G
CRYSTAL STRUCTURE OF URACIL-DNA GLYCOSYLASE FROM T. MARITIMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.25 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.500, 60.500, 127.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.231 |
| Rwork | 0.226 |
| R-free | 0.28080 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 | 0.330 |
| Number of reflections | 8235 | |
| <I/σ(I)> | 39.9 | 8.3 |
| Completeness [%] | 95.6 | 100 |
| Redundancy | 12.8 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 18% PEG4000, 8%isopropanol, HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
