1L9D

Role of Histidine 269 in Catalysis by Monomeric Sarcosine Oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-02
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	71.413, 69.771, 73.169
Unit cell angles	90.00, 92.22, 90.00

Refinement procedure

Resolution	30.000 - 1.950
Rwork	0.171
R-free	0.22800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code 1B3M
RMSD bond length	0.006
RMSD bond angle	23.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.056	0.250
Number of reflections	47553
<I/σ(I)>	22.1	3.4
Completeness [%]	90.4	50.8
Redundancy	4.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	295	phosphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP at 295K, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH8.0
3	1	reservoir	sodium potassium phosphate	1.9-2.1 (M)	pH7.0