1L9C
Role of Histidine 269 in Catalysis by Monomeric Sarcosine Oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-18 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.706, 69.245, 73.324 |
| Unit cell angles | 90.00, 94.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.171 |
| R-free | 0.21600 |
| Structure solution method | refined directly |
| Starting model (for MR) | PDB code 1B3M |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.192 |
| Number of reflections | 50561 | |
| <I/σ(I)> | 13.7 | 3.1 |
| Completeness [%] | 87.7 | 52.5 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 295 | phosphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP at 295K, temperature 295.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | reservoir | sodium potassium phosphate | 1.9-2.1 (M) | pH7.0 |
