1L99
PERTURBATION OF TRP 138 IN T4 LYSOZYME BY MUTATIONS AT GLN 105 USED TO CORRELATE CHANGES IN STRUCTURE, STABILITY, SOLVATION, AND SPECTROSCOPIC PROPERTIES
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.200, 61.200, 95.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 * - 1.950 |
| R-factor | 0.168 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.900 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | 0.092 * |
| Total number of observations | 27921 * |
| Number of reflections | 11881 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 2 | 1 | 1 | 0.1 (M) | or KPO4 | |
| 3 | 1 | 1 | 0.55 (M) | ||
| 4 | 1 | 1 | 0.02 (%) | ||
| 5 | 1 | 1 | phosphate | 1.6-2.2 (M) |
