1L95
SIMILAR HYDROPHOBIC REPLACEMENTS OF LEU 99 AND PHE 153 WITHIN THE CORE OF T4 LYSOZYME HAVE DIFFERENT STRUCTURAL AND THERMODYNAMIC CONSEQUENCES
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.200, 61.200, 95.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.164 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.100 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.068 * |
| Number of reflections | 11126 * |
| Completeness [%] | 77.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | phosphate | 2.0 (M) | |
| 3 | 1 | reservoir | 0.25 (M) | ||
| 4 | 1 | reservoir | beta-mercaptoethanol |
