1L8X
Crystal Structure of Ferrochelatase from the Yeast, Saccharomyces cerevisiae, with Cobalt(II) as the Substrate Ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9678 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.380, 96.660, 120.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hrk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.800 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.174 * | 0.302 |
| Number of reflections | 22973 | |
| <I/σ(I)> | 22.9 | |
| Completeness [%] | 90.9 | 60.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG2000, 2-propanol, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 0.1 (M) | pH8.0 |
| 2 | 1 | drop | glycerol | 20 (%(w/v)) | |
| 3 | 1 | drop | n-octyl-beta-D-glucopyranoside | 1 (%(w/v)) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.5 |
| 5 | 1 | reservoir | PEG2000 | 23 (%(w/v)) | |
| 6 | 1 | reservoir | 2-propanol | 10 (%(v/v)) |
