1L8Q
CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797,1.1272 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 155.752, 155.752, 155.752 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.700 |
| R-factor | 0.23083 |
| Rwork | 0.229 |
| R-free | 0.25500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.061 * | 0.415 * |
| Number of reflections | 17366 | |
| <I/σ(I)> | 15.5 | 5.1 |
| Completeness [%] | 98.6 * | 98.9 * |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | sodium acetate, potassium chloride, hexanediol, PEG MME 2000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 10 | 1 | reservoir | TCEP | 1 (mM) | |
| 11 | 1 | reservoir | 4 (M) | ||
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 400 (mM) | ||
| 4 | 1 | drop | 25 (mM) | ||
| 5 | 1 | drop | ADP | 5 (mM) | |
| 6 | 1 | drop | TCEP | 1 (mM) | |
| 7 | 1 | reservoir | 100 (mM) | pH5.0 | |
| 8 | 1 | reservoir | hexanediol | 14 (%) | |
| 9 | 1 | reservoir | PEG2000 MME | 15 (%) |
