1L8P
Mg-phosphonoacetohydroxamate complex of S39A yeast enolase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2000-09-13 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.200, 164.100, 84.600 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.181 * |
| Rwork | 0.181 |
| R-free | 0.22300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.031 | 0.049 * |
| Total number of observations | 266980 * | |
| Number of reflections | 97095 | |
| Completeness [%] | 96.8 | 96.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 292 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 14 (mg/ml) | |
| 2 | 1 | 1 | 0.26 (M) | ||
| 3 | 1 | 1 | 5 (mM) | ||
| 4 | 1 | 1 | PhAH | 4 (mM) | |
| 5 | 1 | 1 | HEPPS-KOH | 50 (mM) | pH8.0 |
