1L8L

Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-07-20
Detector	MACSCIENCE
Wavelength(s)	0.9794
Spacegroup name	I 4
Unit cell lengths	106.213, 106.213, 87.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 * - 2.500 *
R-factor	0.213
Rwork	0.213
R-free	0.28400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.580
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.660
High resolution limit [Å]	2.500 *	2.510
Rmerge	0.034 *
Total number of observations	82983 *
Number of reflections	15855
Completeness [%]	95.2	75.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	22 *	PEG 6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	sodium potassium phosphate	2.2-2.4 (M)	pH5.0
3	1	reservoir		0.1 (M)
4	1	reservoir	PEG400	2 (%)
5	1	reservoir	dithiothreitol	10 (mM)