1L8L
Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-07-20 |
Detector | MACSCIENCE |
Wavelength(s) | 0.9794 |
Spacegroup name | I 4 |
Unit cell lengths | 106.213, 106.213, 87.812 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 * - 2.500* |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.28400 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.580 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.660 |
High resolution limit [Å] | 2.500 * | 2.510 |
Rmerge | 0.034 * | |
Total number of observations | 82983 * | |
Number of reflections | 15855 | |
Completeness [%] | 95.2 | 75.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 * | 22 * | PEG 6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | sodium potassium phosphate | 2.2-2.4 (M) | pH5.0 |
3 | 1 | reservoir | 0.1 (M) | ||
4 | 1 | reservoir | PEG400 | 2 (%) | |
5 | 1 | reservoir | dithiothreitol | 10 (mM) |