1L7K

x-ray structure of galactose mutarotase from Lactococcus lactis complexed with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	2002-01-26
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	45.200, 76.500, 211.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.950
R-factor	0.157 *
Rwork	0.157
R-free	0.21600
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1l7j
RMSD bond length	0.013
RMSD bond angle	18.800 *
Data scaling software	SAINT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	1.950	1.950
Rmerge	0.063 *	0.225 *
Number of reflections	51652	5042 *
<I/σ(I)>	12	3.4
Completeness [%]	94.3	74.2
Redundancy	2.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	4 *	PEG-5000-O-Methylether, sodium chloride, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000-OMe	15-19 (%)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	drop	enzyme	17.5 (mg/ml)