1L7J

X-ray structure of galactose mutarotase from Lactococcus lactis (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	2002-01-09
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	42.500, 76.100, 206.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.189 *
Rwork	0.189
R-free	0.26700
Structure solution method	MIR
RMSD bond length	0.014
RMSD bond angle	20.500 *
Data scaling software	SAINT
Phasing software	SOLVE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.081 *	0.180 *
Number of reflections	49650	5683 *
<I/σ(I)>	12.2	4.5
Completeness [%]	92.6	86.7
Redundancy	3.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	4 *	PEG-5000-O-Methylether, MES, NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000-OMe	15-19 (%)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	drop	enzyme	17.5 (mg/ml)