1L7I
Crystal Structure of the anti-ErbB2 Fab2C4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.966, 64.249, 79.445 |
| Unit cell angles | 90.00, 105.44, 90.00 |
Refinement procedure
| Resolution | 14.390 - 1.800 |
| Rwork | 0.197 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 27.400 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNX (2000.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.328 |
| Total number of observations | 85734 * | |
| Number of reflections | 36884 | |
| <I/σ(I)> | 5.2 | 1.4 |
| Completeness [%] | 97.6 | 97.6 |
| Redundancy | 2.3 | 2.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 | 292 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 22.5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 35-45 (%sat) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
