1L7I

Crystal Structure of the anti-ErbB2 Fab2C4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-30
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	P 1 21 1
Unit cell lengths	41.966, 64.249, 79.445
Unit cell angles	90.00, 105.44, 90.00

Refinement procedure

Resolution	14.390 - 1.800
Rwork	0.197
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	27.400 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNX (2000.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.065	0.328
Total number of observations	85734 *
Number of reflections	36884
<I/σ(I)>	5.2	1.4
Completeness [%]	97.6	97.6
Redundancy	2.3	2.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	292	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	22.5 (mg/ml)
2	1	reservoir	ammonium sulfate	35-45 (%sat)
3	1	reservoir	Tris-HCl	0.1 (M)	pH8.0