1L6W
Fructose-6-phosphate aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 107 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.490, 126.940, 183.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.930 |
| R-factor | 0.199 * |
| Rwork | 0.198 |
| R-free | 0.21300 |
| Structure solution method | SIR |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.290 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.038 | 0.125 |
| Number of reflections | 200850 * | |
| <I/σ(I)> | 46 | 18 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 20 * | PEG4000, sodium acetate, glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | glycyl-glycine buffer | 50 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | pH8.0 |
| 4 | 1 | reservoir | peg4 | 5.6-9 (%(w/v)) | |
| 5 | 1 | reservoir | sodium acetate | 0.07 (M) | pH4.6 |
| 6 | 1 | reservoir | glycerol | 30 (%(v/v)) |
