1L6O

XENOPUS DISHEVELLED PDZ DOMAIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000,0.9796,0.9795,0.9686
Spacegroup name	P 41 21 2
Unit cell lengths	84.733, 84.734, 123.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.230 - 2.200
R-factor	0.27918
Rwork	0.277
R-free	0.32316
RMSD bond length	0.073
RMSD bond angle	4.597
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	54.230
High resolution limit [Å]	2.200
Number of reflections	21376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Khlebtsova, N., (2000) Acta Crystallogr., D56, 212. *
1	Vapor diffusion, hanging drop *			Khlebtsova, N., (2000) Acta Crystallogr., D56, 212. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	10 (mM)	pH8.
2	1	drop		25 (mM)
3	1	drop	protein	6-8 (mg/ml)
4	1	reservoir	sodium acetate	100 (mM)	pH4.7
5	1	reservoir	sodium formate	1.4 (M)