1L5X
The 2.0-Angstrom resolution crystal structure of a survival protein E (SurE) homolog from Pyrobaculum aerophilum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2002-01-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97870, 0.97860, 0.96485 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 90.500, 90.500, 129.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| Rwork | 0.185 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.790 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.099 | 0.781 |
| Total number of observations | 297069 * | |
| Number of reflections | 42129 | |
| <I/σ(I)> | 17.4 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.55 | 19.8 * | PEG 4000, sodium acetate, glycerol, Tris buffer, pH 8.55, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11.4 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 0.083 (M) | pH8.55 |
| 3 | 1 | reservoir | PEG4000 | 21.7 (%(v/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.17 (M) | |
| 5 | 1 | reservoir | glycerol | 15 (%(v/v)) |
