1L5S
Human liver glycogen phosphorylase complexed with uric acid, N-Acetyl-beta-D-glucopyranosylamine, and CP-403,700
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-06-28 |
Detector | BRANDEIS - B1.2 |
Wavelength(s) | 0.95 |
Spacegroup name | P 31 |
Unit cell lengths | 124.030, 124.030, 123.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.000 * - 2.100 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.23500 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 21.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 * | 0.417 * |
Total number of observations | 243830 * | |
Number of reflections | 117831 * | |
<I/σ(I)> | 9.6 | 1.7 |
Completeness [%] | 94.9 | 75.5 |
Redundancy | 2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 17 * | MES, MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25-30 (mg/ml) | |
2 | 1 | drop | sodium BES | 20 (mM) | pH6.8 |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
5 | 1 | reservoir | sodium MES | 0.1 (M) | pH6.0 |
6 | 1 | reservoir | MPD | 25-35 (%) |