1L5P

Crystal Structure of Trichomonas vaginalis Ferredoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	170
Detector technology	CCD
Collection date	1999-12-22
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	35.400, 64.550, 133.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 * - 2.200
Rwork	0.248
R-free	0.31500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.136
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.070 *
Number of reflections	15472
Completeness [%]	96.0	81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	used microseeding *
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19.0 (mg/ml)
2	1	reservoir	PEG8000	25 (%(w/v))
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)	pH6.5