1L4J
Holliday Junction TCGGTACCGA with Na and Ca Binding Sites.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.073 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.067, 23.775, 73.816 |
| Unit cell angles | 90.00, 110.58, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.850 |
| R-factor | 0.23144 |
| Rwork | 0.228 |
| R-free | 0.29730 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.614 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.062 | 0.199 |
| Number of reflections | 8780 | 1281 * |
| <I/σ(I)> | 14.25 | 5.45 |
| Completeness [%] | 97.3 | 98.4 * |
| Redundancy | 3. * | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | Sodium Cacodylate, Calcium Chloride, MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium Cacodylate | ||
| 2 | 1 | 1 | Calcium Chloride | ||
| 3 | 1 | 1 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 75 (mM) | pH7.0 |
| 2 | 1 | drop | 35 (mM) | ||
| 3 | 1 | drop | 2.5 (%(v/v)) | ||
| 4 | 1 | drop | protein | 1 (mM) | |
| 5 | 1 | reservoir | MPD | 30 (%) |
