1L4I
Crystal Structure of the Periplasmic Chaperone SfaE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X31 |
Temperature [K] | 288 |
Detector technology | AREA DETECTOR |
Collection date | 1995-06-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9204 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.500, 84.240, 97.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.200 |
R-factor | 0.19 * |
Rwork | 0.190 |
R-free | 0.24400 |
Structure solution method | MIR |
RMSD bond length | 0.014 |
RMSD bond angle | 0.037 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.068 * | 0.267 |
Total number of observations | 151034 * | |
Number of reflections | 22920 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.9 | 98.8 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 298 | PEG 4000, sodium citrate, ammonium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
5 | 1 | reservoir | sodium acetate | 0.2 (M) |