1L4A
X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9150 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.072, 75.430, 243.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.950 |
| R-factor | 0.3004 |
| Rwork | 0.297 |
| R-free | 0.34450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 20.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | BEAST |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 * | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.086 | 0.219 |
| Total number of observations | 41657 * | |
| Number of reflections | 10128 | |
| <I/σ(I)> | 4.7 | 3.1 |
| Completeness [%] | 93.6 | 93 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 20 * | 5% MPD, 7.5% PEG550-MME, 20MM beta mercaptoethanol, 20MM Tris PH 7.2, 50MM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | MPD | 4-5.5 (%(v/v)) | |
| 3 | 1 | drop | PEG550 MME | 7-8 (%(w/v)) | |
| 4 | 1 | drop | beta-mercaptoethanol | 20 (mM) | |
| 5 | 1 | reservoir | MPD | 10 (%(w/v)) | |
| 6 | 1 | reservoir | PEG550 MME | 16 (%(w/v)) |
