1L3K

UP1, THE TWO RNA-RECOGNITION MOTIF DOMAIN OF HNRNP A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-01
Detector	BRANDEIS - B4
Spacegroup name	P 1 21 1
Unit cell lengths	37.710, 43.450, 55.060
Unit cell angles	90.00, 93.70, 90.00

Refinement procedure

Resolution	25.780 - 1.100
R-factor	0.146 *
Rwork	0.155
R-free	0.18500 *
Starting model (for MR)	1up1
RMSD bond length	0.008
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.057	0.236
Total number of observations	180601 *
Number of reflections	60334
<I/σ(I)>	11
Completeness [%]	83.6	52
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	289 *	Jokhan, L., (1997) Acta Crystallogr., D53, 615. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		300 (mM)
3	1	drop	MES	20 (mM)	pH6.0
4	1	drop	EDTA	1 (mM)
5	1	drop	beta-mercaptoethanol	0.1 (%)
6	1	reservoir	PEG4000	25 (%)
7	1	reservoir	MPD	20 (%)