1L3K
UP1, THE TWO RNA-RECOGNITION MOTIF DOMAIN OF HNRNP A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-01 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.710, 43.450, 55.060 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 25.780 - 1.100 |
| R-factor | 0.146 * |
| Rwork | 0.155 |
| R-free | 0.18500 * |
| Starting model (for MR) | 1up1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.057 | 0.236 |
| Total number of observations | 180601 * | |
| Number of reflections | 60334 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 83.6 | 52 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 289 * | Jokhan, L., (1997) Acta Crystallogr., D53, 615. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | MES | 20 (mM) | pH6.0 |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 0.1 (%) | |
| 6 | 1 | reservoir | PEG4000 | 25 (%) | |
| 7 | 1 | reservoir | MPD | 20 (%) |
