1L3J
Crystal Structure of Oxalate Decarboxylase Formate Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.948 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 154.800, 154.800, 122.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.720 - 1.900 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.720 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 0.260 |
| Number of reflections | 44171 | |
| <I/σ(I)> | 7.1 | 7.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | PEG 4000, formate,Tris, sodium chloride, pH 8.0, VAPOR DIFFUSION, temperature 298K |
