1L3C
MT0146, THE PRECORRIN-6Y METHYLTRANSFERASE (CBIT) HOMOLOG FROM M. THERMOAUTOTROPHICUM, C2 SPACEGROUP WITH SHORT CELL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97956 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.300, 59.600, 98.700 |
Unit cell angles | 90.00, 113.90, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.300* |
Rwork | 0.204 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f38 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.520 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 * | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.059 * | 0.191 |
Number of reflections | 49834 | |
<I/σ(I)> | 4.8 | |
Completeness [%] | 88.1 | 79.7 |
Redundancy | 2.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 25 * | PEG 8000, MgCl, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | drop | PEG8000 | 10 (%(w/v)) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | Tris-Cl | 5 (mM) | pH7.5 |
5 | 1 | reservoir | PEG8000 | 16-22 (%) | |
6 | 1 | reservoir | 200 (mM) | ||
7 | 1 | reservoir | Tris-Cl | 10 (mM) | pH7.5 |