1L39

CONTRIBUTIONS OF ENGINEERED SURFACE SALT BRIDGES TO THE STABILITY OF T4 LYSOZYME DETERMINED BY DIRECTED MUTAGENESIS

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	61.100, 61.100, 96.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.850
R-factor	0.151
RMSD bond length	0.017
RMSD bond angle	0.016 *
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.850 *
Rmerge	0.065 *
Number of reflections	12838 *
Completeness [%]	69.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *			taken from Remington, S.J. et al (1978). J. Mol. Biol., 81-98. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		0.55 (M)
3	1	1	mercaptoethanol	14 (mM)
4	1	1		1 (mM)
5	1	1	sodium phospahte	0.01 (M)
6	1	1		2.2 (M)
7	1	1		1.8 (M)