1L2U
Orotidine 5'-monophosphate decarboxylase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 99.9 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0159 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 142.690, 142.690, 105.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.162 |
| Rwork | 0.159 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eix |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.091 * | 0.470 |
| Number of reflections | 42181 * | |
| <I/σ(I)> | 19 | 3.5 |
| Completeness [%] | 99.3 * | 99 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 18 * | sodium formate, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 2.5 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.5 |
