1L2T
Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-01 |
| Detector | BRANDEIS - B1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.887, 106.311, 116.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.185 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.114 * | |
| Number of reflections | 39885 | |
| Completeness [%] | 100.0 * | 87.8 |
| Redundancy | 6.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 295 | PEG 4000, Sodium Hepes, Isopropanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18 (%(w/v)) | |
| 2 | 1 | reservoir | isopropanol | 10 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.0 |
| 4 | 1 | drop | protein | 3 (mg/ml) | |
| 5 | 1 | drop | sodium-EDTA | 1 (mM) | |
| 6 | 1 | drop | glycerol | 10 (%) | |
| 7 | 1 | drop | dithiothreitol | 4 (mM) | |
| 8 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
