1L1S

Structure of Protein of Unknown Function MTH1491 from Methanobacterium thermoautotrophicum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-14
Detector	CUSTOM-MADE
Wavelength(s)	0.93927
Spacegroup name	P 63
Unit cell lengths	82.440, 82.440, 37.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.550 - 2.300
R-factor	0.202
Rwork	0.202
R-free	0.22600
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	23.200 *
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.071	0.183 *
Total number of observations	77390 *
Number of reflections	6492 *
<I/σ(I)>	32.6	7.6
Completeness [%]	98.4 *	99.8
Redundancy	12	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	20 *	30 % MPD, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	30 (%)
2	1	1	HEPES	100 (mM)	pH7.5