1L1L
CRYSTAL STRUCTURE OF B-12 DEPENDENT (CLASS II) RIBONUCLEOTIDE REDUCTASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-28 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.1022 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.500, 114.700, 121.300 |
| Unit cell angles | 90.00, 110.20, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 1.750 |
| R-factor | 0.223 * |
| Rwork | 0.223 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.570 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.058 * | 0.267 * |
| Total number of observations | 632497 * | |
| Number of reflections | 285688 | |
| <I/σ(I)> | 13.5 | 2.1 |
| Completeness [%] | 91.6 | 60.2 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | ammonium sulfate, PEG 8000, glycerol, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 50 (mM) | pH5.6 |
| 3 | 1 | drop | glycerol | 20 (%(v/v)) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 7 | 1 | reservoir | PEG8000 | 2 (%(v/w)) | |
| 8 | 1 | reservoir | glycerol | 20 (%(v/v)) | |
| 9 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
