1L1J

Crystal structure of the protease domain of an ATP-independent heat shock protease HtrA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 3
Unit cell lengths	120.550, 120.550, 120.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.228
R-free	0.28400 *
RMSD bond length	0.008 *
RMSD bond angle	1.530 *
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.066 *	0.270 *
Number of reflections	13843 *	1376 *
Completeness [%]	95.1 *	97.2 *
Redundancy	2.43 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	22 *	phosphate-citrate, Li2SO4, PEG1000, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	phosphate-citrate	100 (mM)	pH4.4
2	1	reservoir		110 (mM)
3	1	reservoir	PEG1000	5 (%(v/v))