Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.65 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.590, 138.090, 275.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.245 * |
| Rwork | 0.245 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| Starting model (for MR) | 1FUM |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.093 * | 0.277 * |
| Total number of observations | 219456 * | |
| Number of reflections | 49332 | |
| <I/σ(I)> | 15.5 | 6.5 |
| Completeness [%] | 87.2 | 90 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 * | 298 | PEG 10000, MgAcetate, NaCitrate, DTT, EDTA, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | polyoxyethylene getergent C12E9 | 0.7 (%(w/v)) | |
| 3 | 1 | reservoir | PEG5000 MME | 12.5 (%) | |
| 4 | 1 | reservoir | magnesium acetate | 85 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.8 |
| 6 | 1 | reservoir | EDTA | 0.100 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 0.001 (%) |
