1KZB
Complex of MBP-C and trimannosyl core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.720, 75.330, 57.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.800 |
| R-factor | 0.217 |
| Rwork | 0.212 |
| R-free | 0.25900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1rdo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.111 |
| Number of reflections | 23828 | |
| <I/σ(I)> | 17.3 | 4 |
| Completeness [%] | 94.8 | 81.8 |
| Redundancy | 3.5 * | 1.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20-22 * | PEG 8000, Tris-Cl, NaCl, CaCl2, NaN3, pH 7.4, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-20 (mg/ml) | |
| 2 | 1 | drop | Tris-Cl | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 12 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 11-14 (%) | |
| 5 | 1 | reservoir | Tris-Cl | 100 (mM) | pH7.4 |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | 12 (mM) | ||
| 8 | 1 | reservoir | 2 (mM) |
