1KZ1
Mutant enzyme W27G Lumazine Synthase from S.pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 295 |
| Detector technology | CCD |
| Collection date | 2001-04-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.568, 144.017, 126.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.590 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 * | 0.750 * |
| Number of reflections | 66756 | |
| Completeness [%] | 98.0 | 98 * |
| Redundancy | 3.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 295 | citrate, ammonium dihydrogen phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 20 (mM) | pH7.0 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | citrate | 0.1 (M) | pH4.9-5.2 |
| 5 | 1 | reservoir | sodium formate | 1.5 (M) |
