1KYT

Crystal Structure of Thermoplasma acidophilum 0175 (APC014)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	150
Detector technology	CCD
Collection date	2001-11-30
Detector	SBC-2
Wavelength(s)	1.0719
Spacegroup name	I 2 2 2
Unit cell lengths	88.526, 99.316, 113.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.140 - 1.700
R-factor	0.226
Rwork	0.224
R-free	0.24600
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	CrystalClear (D*STREK (MSC/RIGAKU))
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.140	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.074	0.244
Number of reflections	53243
<I/σ(I)>	7.4	2.6
Completeness [%]	97.4	81.6
Redundancy	5.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350, sodium HEPES, Calcium Chloride, Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350, sodium HEPES, Calcium Chloride, Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 3350, sodium HEPES, Calcium Chloride, Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K