1KY8

Crystal Structure of the Non-phosphorylating glyceraldehyde-3-phosphate Dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-04
Detector	MARRESEARCH
Wavelength(s)	0.8469
Spacegroup name	P 62 2 2
Unit cell lengths	185.200, 185.200, 132.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
Rwork	0.208
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.480
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.005 *	0.470 *
Total number of observations	792801 *
Number of reflections	53177 *
Completeness [%]	99.2 *	99.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	sodium formate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	sodium formate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	sodium formate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	sodium formate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	sodium formate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	2.0 (M)