1KY7

THE AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH AMPHIPHYSIN FXDXF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-06-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	40.630, 73.690, 41.940
Unit cell angles	90.00, 99.74, 90.00

Refinement procedure

Resolution	19.900 - 2.150
R-factor	0.187 *
Rwork	0.187
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qts
RMSD bond length	0.010
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.150	2.150
Rmerge	0.071 *	0.404 *
Total number of observations	41702 *
Number of reflections	11788
<I/σ(I)>	13.8	2.6
Completeness [%]	88.6	88.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein/peptide solution	6 (mg/ml)
2	1	reservoir	ammonium sulfate	1.36-1.49 (M)
3	1	reservoir	HEPES	85-93 (mM)	pH6.8
4	1	reservoir	dioxane	8.5-9.3 (%)
5	1	reservoir	glycerol	0-5 (%)