1KY6

AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH EPSIN DPW PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-10-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	37.302, 40.896, 41.611
Unit cell angles	99.28, 96.42, 109.47

Refinement procedure

Resolution	19.790 - 2.000
R-factor	0.186 *
Rwork	0.186
R-free	0.24600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qtp
RMSD bond length	0.010
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.061 *	0.181 *
Total number of observations	52333 *
Number of reflections	15307
<I/σ(I)>	14.4	3.6
Completeness [%]	88.9	62.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein/peptide solution	6 (mg/ml)
2	1	reservoir	ammonium sulfate	1.36-1.49 (M)
3	1	reservoir	HEPES	85-93 (mM)	pH6.8
4	1	reservoir	dioxane	8.5-9.3 (%)
5	1	reservoir	glycerol	0-5 (%)