1KY3

GDP-BOUND YPT7P AT 1.35 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-10
Detector	MARRESEARCH
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	49.535, 55.430, 60.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.610 - 1.350
R-factor	0.201 *
Rwork	0.161
R-free	0.25300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	YPT7-GPPNHP
RMSD bond length	0.014 *
RMSD bond angle	2.130 *
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.450
High resolution limit [Å]	1.350	1.300 *
Rmerge	0.081 *	0.308
Total number of observations	263110 *
Number of reflections	36543
<I/σ(I)>	15.77	4.33
Completeness [%]	98.6	97.3
Redundancy	5.4	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	PEG, HEPES, GDP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-9 (mg/ml)
2	1	reservoir	PEG3350	20 (%)
3	1	reservoir		200 (mM)