1KY3
GDP-BOUND YPT7P AT 1.35 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.535, 55.430, 60.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.610 - 1.350 |
| R-factor | 0.201 * |
| Rwork | 0.161 |
| R-free | 0.25300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | YPT7-GPPNHP |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 2.130 * |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.300 * |
| Rmerge | 0.081 * | 0.308 |
| Total number of observations | 263110 * | |
| Number of reflections | 36543 | |
| <I/σ(I)> | 15.77 | 4.33 |
| Completeness [%] | 98.6 | 97.3 |
| Redundancy | 5.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG, HEPES, GDP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-9 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 20 (%) | |
| 3 | 1 | reservoir | 200 (mM) |
