1KXZ
MT0146, the Precorrin-6y methyltransferase (CbiT) homolog from M. Thermoautotrophicum, P1 spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.100, 76.000, 97.200 |
| Unit cell angles | 70.90, 78.80, 66.70 |
Refinement procedure
| Resolution | 40.000 * - 2.640* |
| Rwork | 0.212 |
| R-free | 0.25720 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f38 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.770 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 2.770 |
| High resolution limit [Å] | 2.640 * | 2.700 |
| Rmerge | 0.150 * | |
| Number of reflections | 39161 | |
| Completeness [%] | 98.8 | 97.3 * |
| Redundancy | 1.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 25 * | PEG8000, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 10 (%(w/v)) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | Tris-Cl | 5 (mM) | pH7.5 |
| 5 | 1 | reservoir | PEG8000 | 16-22 (%) | |
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | Tris-Cl | 10 (mM) | pH7.5 |
