1KXQ

Camelid VHH Domain in Complex with Porcine Pancreatic alpha-Amylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-10-15
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	65.170, 100.920, 103.740
Unit cell angles	79.31, 72.63, 86.11

Refinement procedure

Resolution	30.000 * - 1.600
R-factor	0.197 *
Rwork	0.197
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jfh PDB ENTRY 1QD0
RMSD bond length	0.009
RMSD bond angle	25.100 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.600	1.650 *
Rmerge	0.064	0.322
Total number of observations	3531130 *
Number of reflections	383526
<I/σ(I)>	5	1.7
Completeness [%]	99.8 *	99.5 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	293	Phosphate buffer 0.8 M, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	50 (mM)	pH7.5
2	1	drop		100 (mM)
3	1	reservoir	phosphate buffer	0.8 (M)	pH7.0