1KXQ
Camelid VHH Domain in Complex with Porcine Pancreatic alpha-Amylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.170, 100.920, 103.740 |
| Unit cell angles | 79.31, 72.63, 86.11 |
Refinement procedure
| Resolution | 30.000 * - 1.600 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jfh PDB ENTRY 1QD0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.650 * |
| Rmerge | 0.064 | 0.322 |
| Total number of observations | 3531130 * | |
| Number of reflections | 383526 | |
| <I/σ(I)> | 5 | 1.7 |
| Completeness [%] | 99.8 * | 99.5 * |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | Phosphate buffer 0.8 M, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | reservoir | phosphate buffer | 0.8 (M) | pH7.0 |
