1KXI

STRUCTURE OF CYTOTOXIN HOMOLOG PRECURSOR

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-09-25
Detector	RIGAKU RAXIS II
Spacegroup name	P 41 21 2
Unit cell lengths	43.300, 43.300, 147.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.190
R-factor	0.207
Rwork	0.207
R-free	0.30500
RMSD bond length	0.010
RMSD bond angle	1.600
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.190 *
Rmerge	0.080
Total number of observations	38418 *
Number of reflections	7280
Completeness [%]	91.7	82.2 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG4000	10 (%)
3	1	drop		0.15 (M)
4	1	drop	Tris-HCl	0.1 (M)
5	1	reservoir	PEG4000	20 (%)