1KXD

SINDBIS VIRUS CAPSID (N222L MUTANT), TETRAGONAL CRYSTAL FORM

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-12-31
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	57.000, 57.000, 109.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.179
Rwork	0.179
RMSD bond length	0.010
RMSD bond angle	27.500 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.054	0.137 *
Total number of observations	11795 *
Number of reflections	4040
Completeness [%]	85.7	69 *
Redundancy	2.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3-4 (mg/ml)
2	1	drop	PEG8000	3-4 (%(w/v))
3	1	drop	Tris-HCl	25-150 (mM)
4	1	reservoir	PEG8000	6-8 (%(w/v))
5	1	reservoir	Tris-HCl	50-300 (mM)