1KXB

SINDBIS VIRUS CAPSID (S215A MUTANT), TETRAGONAL CRYSTAL FORM

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-09-23
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	57.000, 57.000, 109.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.900
R-factor	0.184
Rwork	0.184
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.048	0.112 *
Total number of observations	11551 *
Number of reflections	4335
Completeness [%]	91.9	79.4 *
Redundancy	2.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.5-7.5 (mg/ml)
2	1	reservoir	PEG8000	6-8 (%(w/v))
3	1	reservoir	Tris-HCl	50-300 (mM)