1KXA

SINDBIS VIRUS CAPSID, (WILD-TYPE) RESIDUES 106-264, TETRAGONAL CRYSTAL FORM

Experimental procedure

Source type	SEALED TUBE
Detector technology	AREA DETECTOR
Collection date	1994-07-03
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	57.000, 57.000, 109.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.100
R-factor	0.174
Rwork	0.174
RMSD bond length	0.010
RMSD bond angle	27.600 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.076	0.237 *
Total number of observations	8276 *
Number of reflections	3596 *
Completeness [%]	76.3	27.1 *
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	6-8 (%(w/v))
2	1	reservoir	Tris-HCl	50-300 (mM)
3	1	drop	protein	2.25-3.75 (mg/ml)
4	1	drop	PEG8000	3-4 (%(w/v))
5	1	drop	Tris-HCl	25-150 (mM)