1KWM
Human procarboxypeptidase B: Three-dimensional structure and implications for thrombin-activatable fibrinolysis inhibitor (TAFI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.959, 83.558, 127.447 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 27.400 - 1.600 |
| R-factor | 0.136 |
| Rwork | 0.135 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.670 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.050 * | 0.241 * |
| Total number of observations | 303465 * | |
| Number of reflections | 104805 | |
| <I/σ(I)> | 8.4 | 2.7 |
| Completeness [%] | 89.7 | 81.9 |
| Redundancy | 2.9 * | 2.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 20 * | MES, sodium citrate, sodium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | MOPS | 5 (mM) | pH7.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | benzamidine | 0.1 (mM) | |
| 5 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
| 6 | 1 | reservoir | sodium citrate | 1 (M) |
