1KWF
Atomic Resolution Structure of an Inverting Glycosidase in Complex with Substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8815 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.336, 62.767, 103.493 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 0.940 |
| R-factor | 0.094 |
| R-free | 0.11300 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.950 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Rmerge | 0.043 | 0.190 |
| Total number of observations | 903691 * | |
| Number of reflections | 208140 | |
| Completeness [%] | 99.8 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | used microseeding, Souchon, H., (1996) PROTEINS: STRUCT.,FUNCT.,GENET., 25, 134. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 0.1 (M) | pH7.5 |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | drop | PEG4000 | 17 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.2 |
| 5 | 1 | reservoir | PEG8000 | 15 (%) |
