1KW4

Polyhomeotic SAM domain structure

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Detector technology	CCD
Collection date	2001-04-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.969575, 0.97855, 0.979224
Spacegroup name	P 65
Unit cell lengths	59.836, 59.836, 44.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 1.750
R-factor	0.22
Rwork	0.220
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.160 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.750	1.750
Rmerge	0.038 *	0.194 *
Total number of observations	224372 *
Number of reflections	17395
Completeness [%]	99.1 *	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	20 *	Li2SO4, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16-20 (mg/ml)
2	1	reservoir	sodium citrate	50 (mM)	pH4.0
3	1	reservoir		1.9 (M)